Return to HomepageRichard Mazzarella, Ph.D.
Education and Skills
EDUCATION
B.S. BIOLOGY 1980 LOYOLA MARYMOUNT UNIVERSITY, LOS ANGELES, CA
Ph.D. Microbiology 1987 Saint Louis UNIVERSITY, ST LOUIS, MO
PROGRAMMING LANGUAGES
C/C++
PERL
PYTHON
JAVA
JAVASCRIPT
SQL and PL/SQL
HTML
SKILLS AND JOB ACCOMPLISHMENTS
Identification of Human Disease Targets (Searle/Pharmacia/Pfizer)
§ Study of Angiogenesis and Cancer
§ Study of Osteoarthritis
§ Study of Hypertension & Syndrome X/Metabolic Syndrome
1999-2007
Identification of colon cancer antibody targets (resulted in a Phase I Start)
Identification osteoarthritis candidate genes (resulted in a Phase II OA Pain Start and a Phase I General Pain Start)
Identification of protein sequence motifs recognized and utilized by ADAMTS-4 & ADAMTS-5
Identification of genes involved in breast cancer pathology
Identification of genes involved in angiogenesis
Identification of a novel chemokine (CXCL17)
Analysis of linkage data for genes involved in Osteoarthritis
Prediction of the cellular function of unknown disease candidate genes
Analysis of association data for genes involved in Metabolic Syndrome
Analysis of microarray data (Agilent and Affymetrix)
Analysis of large amounts of cDNA sequence information from model systems
Text mining of literature information
Analysis of pathway information
Development of tools for data access for bench scientists
Maintenance of web server with tools for target discovery
The Human Genome Project (HGP) - Mapping, Sequencing and Analysis of the Human X Chromosome (Washington University)
§ Joined HGP on First Official Day
§ Member of Group that Assembled Map of Entire Human X Chromosome
§ Identification and Analysis of Human Disease Loci
§ Assembly and Analysis of Human Genomic Sequence
§ Gene Prediction and Prediction of Protein Function and Subcellular Localization
§ PNAS paper sponsored by Nobel Laureate Jim Watson (HGP Head)
1991 - 1999
Isolation of very high molecular weight genomic DNA
Construction of genomic yeast artificial chromosome (YAC) libraries
Polymerase chain reaction application
Pulsed-field gel electrophoresis of yeast chromosomes and YACs
Restriction enzyme mapping of genomic DNA
Analysis of genomic sequence information
Genome mapping
Phylogenetic mapping
CpG island analysis
Gene and isoform prediction
Analysis of genome repetitive elements
Analysis of recombination events resulting in human pathology
Analysis of linkage data
Prediction of gene function
Prediction of the subcellular localization of hypothetical proteins
Identification of X-linked genes involved in human disease
Identification, Characterization and Cloning of Endoplasmic Reticulum (ER) Proteins Saint Louis University
§ Study of Lymphocyte Differentiation
§ Identification, Characterization and Cloning of 4 ER Stress Proteins
§ Study of ER Biogenesis
§ Study of ER Protein Sorting
§ Strong Immunology, Biochemistry and Molecular Biology Training
1981 - 1990
Plaque, RIA and ELISA assays
LPS-induced differentiation of lymphocytes
Subcellular fractionation of organelles by sucrose gradients
Preparation of highly purified ER
Purification of ER proteins of column chromatography
Maintenance of plasmacytomas and ascites tumors in mice
Antibody production in mice, rats and rabbits using highly purified proteins
Production of monoclonal antibody cell lines
Design of an immunoassay for antibodies to the ER
Maintenance of monoclonal antibody cell lines in nude mice
Northern, Southern and Western blot analysis
Purification of mRNA from plasmacytoma cell lines
Fractionation of mRNA by sucrose gradients
Reconstitution of cell-free protein synthesis from ascites tumors
Cell-free protein synthesis by rabbit reticulocyte lysate
Immunoprecipitation of specific ER proteins
Protein polyacrylamide gel electrophoresis
Construction of cDNA libraries
Isolation of cDNA clones
Sanger DNA sequencing
DNA and RNA agarose gel electrophoresis
N-terminal protein sequencing by Edman degradation
Computational assembly of DNA sequences
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